Composite

Part:BBa_K2444013:Design

Designed by: Ian Pierce   Group: iGEM17_Delaware   (2017-10-27)


Codes for CvaA/B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1740
    Illegal EcoRI site found at 2407
    Illegal PstI site found at 668
    Illegal PstI site found at 881
    Illegal PstI site found at 1758
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1740
    Illegal EcoRI site found at 2407
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 668
    Illegal PstI site found at 881
    Illegal PstI site found at 1758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1740
    Illegal EcoRI site found at 2407
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1740
    Illegal EcoRI site found at 2407
    Illegal PstI site found at 668
    Illegal PstI site found at 881
    Illegal PstI site found at 1758
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1740
    Illegal EcoRI site found at 2407
    Illegal PstI site found at 668
    Illegal PstI site found at 881
    Illegal PstI site found at 1758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1374
    Illegal SapI site found at 2483


Design Notes

a complete construct with indicator, restriction enzymes sites, and promoter for bacteriocin expression


Source

E. coli

References